Objective To study the effect of the traditional Chinese medicine Jiaweiwuru Tang on calcium oxalate renal stone formation induced by ethylene glycol(EG) and vitamin D_3([Ia(OH)VitD3]) in Sprague-Dawley rats.(Jiaweiwuru Tang is composed of Qiancaogen,Wuzeigu,Beiqi,Rougui,Jinqiancao,Haijinsha,etc.) Methods The renal function,calcium oxalated crystallization,24h urinary calcium and oxalate excretion were measured.The kidney tissues were examined by histopathology.Results In the Jiaweiwuru Tang treatment groups the renal tissue calcium and urinary calcium excretion were significantly lower than those in model groups,and there was few oxalate crystals precipitated in the renal tissue.Conclusion Jiaweiwuru Tang is effective in preventing the formation of calcium oxalate stones by inhibiting calcium oxalated crystals growth and aggregation.