Objective To analyse SNP(single nucleotide polymorphism) loci of the domestic inbred mouse gene by the lately established method(single_tube bi_directional allele specific amplification(SB_ASA)).Method Four domestic inbred mouse strains were detected by 16 SNP loci with the method of SB_ASA,and sequencing was designed to validate the reliability of the method.Results SB_ASA was successfully used to type 4 inbred mouse strains for 16 SNP loci,and the results were completely consistent with sequencing.Conclusion SB_ASA could be used to exactly inspect genotype of the domestic inbred mouse.