Objective In order to promote the cultivation and establishment of new inbred mice, the heredity genes of baby mice were monitored by microsatellites DNA and selectively breeding in this experiment. Methods DNA polymorphism in baby mice between inbred C57 and BALB/c mice was assessed by microsatellites DNA with PCR amplification. The inbred mice were bred using high and moderate similarity coefficient of female C57 mice of Fo . Results There were 14 polymorphism loci for F2 mice, and rate of homo-gene was 53.3 % . There were 29 homo-gene loci in F,o mice, and rate of homo-gene loci was 96.7 % . The similarity coefficient of mice in the sequential generations was gradually increasing by 3% - 10% from generation to generation. The fact that the mice of F,o generation are inbred mice, was verified and proved by skin transplantation. Conclusion A new method of fast cultivation of inbred mice has been established, therefore, much improved the traditional inbred cultivation techniques. It may considerably facilitate researches on blood relationship and genetic distance in animals in future.