Along with the conversion of medicine research pattern, the model research for replacing animals experiment has become an important aspect of toxicology development. Presently, the substitute methods for cosmetics toxicity test were investigated from monolayer cell culture, organ culture and chicken embryo culture run into artificial organ reconstruct in 3- dimensions. A good few of products have been provided merchandizing. Numbers of methods has been validating by relevant institution and application by EU and USA. Therefore, exploiting new replacement products and methods for cosmetics safety evaluation is not only the bulwark technique problem that inspection and quarantine must be replying but also the requirement of development of laboratory animal sciences.