目的建立检测兔出血症病毒的RT-PCR方法。方法自感染RHDV致死的兔肝脏组织中提纯病毒，提取病毒总RNA，以RT-PCR方法扩增出368bp的基因片段。将RT-PCR产物克隆到T载体中，并对重组质粒测序。结果PCR产物的测序结果与文献报道的序列一致，敏感性试验结果表明，可检测到10^-7病毒滴度。结论建立的检测RHDVRT-PCR方法具有很高的特异性和敏感性。
Objective To establish a RT-PCR method for detection of rabbit hemorrhagic disease virus (RHDV).Methods RHDV were purified from liver samples of rabbits died from RHDV infection,and total RNA was prepared.A 368 bp fragment was amplified by RT-PCR,then was cloned into pMEG-T vector.The recombinant plasmid was sequenced.Results The sequencing result of PCR products was homologous with the nucleotide sequence reported in the reference.The sensitivity assays indicated that 10~(-7) virus titer can be detected by PCR.Conclusion The established RT-PCR method for detection of RHDV is highly sensitive and specific.
巩薇,付瑞,贺争鸣,邢瑞昌.兔出血症病毒RT-PCR方法的研究[J].中国比较医学杂志,2006,(8):466~468.
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