高脂饲料对C57BL/6J小鼠血脂及转氨酶的动态影响
中图分类号:

R542.22

基金项目:

国家自然科学基金;北京市自然科学基金


Dynamic Observation of Serum Lipid and ALT Levels in C57BL/6J Mouse Models of Hyperlipidemia Induced by High-Lipid Diet
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    摘要:

    目的观察高脂饲料诱发的C57BL/6J小鼠高脂血症模型血脂的动态变化。方法C57BL/6J小鼠分成基础饲料组、高脂饲料组,在给予高脂饲料0、1、2、3、4、5、7、9、11、17周采血检测TC、TG、HDL-C、LDL-C、ALT、BUN,并且部分动物取心、肝、肾甲醛液固定,HE染色作病理学检查,剩余部分动物继续给予普通饲料,21周时采血检测血浆指标。结果给予高脂饲料后1周小鼠血脂和ALT显著升高,3~11周后稳定,此后稍有下降。BUN水平没有明显变化。心、肝脏显示明显病理学损伤,肾脏病理学检测正常。但是停止饲喂高脂饲料,小鼠血脂和ALT水平很快下降至正常。结论高脂饲料引起C57BL/6J小鼠血脂升高迅速、明显。

    Abstract:

    Objective To establish a hyperlipidemia animal model induced by high-fat diet,and observe the dynamic variation of the serum lipid levels at different time.Methods The C57BL/6J mice were randomly divided into 2 groups.The control group was fed with standard diet,the model group fed with high-fat diet.The serum TG,TC,HDL,LDL,ALT,albumin,and BUN were assayed at 0,1,2,3,4,5,7,9,1 1,17 weeks.Some mice were killed and the heart,liver and kidney were taken and HE-stained sections were prepared for histopathological examination.Then high-fat diet of the model group was changed to standard diet.After 4 weeks all mice were killed and the serum contents of each substance were detected.Results The levels of lipid and ALT in serum rose rapidly after 1 week of high-fat diet,and stay at a high level throughout the duration of 3-11weeks,then decreased a little.Pathological changes were observed in the heart and liver.The serum lipid and ALT levels decreased rapidly to normal level after stopping high-fat diet.Conclusion Feeding with high-fat diet can induce hyperlipidemia in mice rapidly and reliably.

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王丽娟,李晓蓉,李宇航,徐艳霞,薛明.高脂饲料对C57BL/6J小鼠血脂及转氨酶的动态影响[J].中国比较医学杂志,2007,(10):572~575,I0003.

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  • 最后修改日期:2007-06-27
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