Objective To establish a method for purification of IgG from tiger serum with high purity. Methods The tiger serum was subject to precipitation with ammonium sulfate and then purified by Protein-A Affinity Chromatography and anion exchange chromatography,The purity of IgG was identified by SDS-PAGE and Western-Blot methods.Results Eighty-four mg IgG was purified from 80 mL tiger serum using Protein-A Affinity Chromatography and Thirty mg IgG was further purified from the 84mg affinity purification IgG using anion exchange chromatography.Conclusion The method was established for purification of tiger IgG,and provide IgG antigens for the production of polyclonal rabbit anti-tiger-IgG secondary antibody.