R-33
中央级科研所科技基础性工作专项重点项目;黑龙江省科技攻关计划
目的测定不同周龄的BWEL-SPF种鸡生理和生化指标,并对测定值进行t-检验。方法分别采用计数板法、血涂片法、血液全自动分析仪测定法、双缩尿法、溴甲酚绿法、酶比色法和硫氰酸汞比色法等测定了10、40和60周龄的雌、雄BWEL-SPF种鸡的生理生化指标,并对雌、雄差异进行了统计学分析。结果在所测定的生理生化指标中,不同周龄雌雄之间差异显著性表现项目不同,其中在10周龄时,只有总胆固醇(CHO)在雌、雄之间差异极显著(P<0.01);40周龄时,甘油三酯(TG)、在雌、雄之间差异极显著(P<0.01);总胆固醇(CHO)为雌、雄之间差异显著(P<0.05);60周龄时,出血时间(BT)、嗜碱性粒细胞(B)、单核细胞(M)、谷丙转氨酶(ALT)、葡萄糖(Glu)在雌、雄之间差异极显著(P<0.01),而凝血时间(CT)和甘油三酯(TG)在雌、雄之间差异显著(P<0.05),除此以外,其它生理生化指标在雌雄之间差异不显著。结论BWEL-SPF种鸡的生理和生化指标随周龄不同,在雌、雄之间的差异随之变化。
Objective To determine the values of physiological and biochemical parameters in BWEL-SPF chickens at age of 10,40 and 60 weeks.Methods The values of T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,Alb,AST,ALT,Glu and TG and CHO in BWEL-SPF chickens at age of 10,40 and 60 weeks,both male and female,were determined by corresponding measurement and t-test was made to analyze the results.Results There was a significant difference between CHO values in male and female chickens at age of 10 weeks(P<0.01),a very significant difference between TG,Ca,Na and Mg values in male and female chickens at age of 40 weeks(P<0.01),a significant difference between CHO values in male and female chickens at age of 40 weeks(P<0.05),a very significant difference between BT,B,M,ALT and Glu values in male and female chickens at age of 60 weeks(P<0.01),and a significant difference between CT and TG values in male and female chickens at age of 60 weeks(P<0.05).Conclusion The values of the physiological and biochemical parameters were different between male and female BWEL-SPF chickens at age of 10,40 and 60 weeks.
冷超,韩凌霞,于海波,栾颖,李淑兰,曲连东.不同周龄BWEL-SPF种鸡生理生化指标的测定[J].中国比较医学杂志,2007,(12):697~701.
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