In recent years, animal models of hemorrhoids have been successfully established in many species such as rabbits, rats, mice and dogs, induced by croton oil, acetic acid, infection, and wound. Each animal model has its advantages and disadvantages. Using reasonable animal models may make it better to carry on experimental and clinical studies of hemorrhoids. Along with the research and production of hemorrhoids drugs, the animal models are well developed. In this paper, the methods of preparation of animal models of hemorrhoids were reviewed.