Objective To detect the genetic stability in four elonal cell strains of murine hepatoma 22 with RAPD analysis. Methods Genomic DNAs from H22-H8D8,H22-H2G4,H22-H2E10,and H22-H2C4 were amplified by RAPD with 47 10bp random primers. Results The most amplified products were monophic, 4 of the 47primers generated polymorphic profile in four clonal cell strains. A muhiband profile were observed for all the primers with the totle number of bands per primer varying from 4 to 10 mostly, and these bands ranged from 200bp to 2kb. The alterations exhibited in the four conal cell lines included the loss of bands, shift of bands and intensity changes of bands. Conclusion Difference in genomic DNAs of four conal cell strains have been detected by RAPD analysis.