Objective To infect mouse by various paths and study the path to monitor bacterial virulence. Methods Injected mitomycin into mouse and injected Streptococcus suis type 2, EHEC O26 by wound, douching stomach, abdominal cavity. Used Enterococcus faecalis ATCC 29212 and Escherichia coil ATCC25922 as negative control. Observated clinicaling and determinated microbiolog. Results The falallty rate of the mouse that infected by douching stomach is 60 % , the fatality rate of the mouse that infected by wound is 60% . We can isolate Streptococcus suis type 2 from the dead mouse of the puncturing of heart. None of mouse that had been injected Streptococcus suis type 2 and Enterococvusfaecalis ATCC 29212 by abdominal cavity dead, hut the mouse that had been injected EHEC O26 and Escherichia coli ATCC25922 dead within 10 hotws. Conclusion In our study, we inoculated bacteria in the mouse proximal tails that scarified by artificial, then the mouse infected Streptococcus suis type 2. So, we confnrmed the path that infected Streptococcus suis type 2 by wound, we simultaneously detected that injecting Escherchia coli and other Gram negative Bacilli will cause non-specific death. It has an important significance for studying the mechanism of the pathogenicity for Streptococcus suis type 2, manufacturing and evaluating the vaccine and observing the effect of antiseptic drug.