Cryobiology is a jumped-up subject which investigates the influence of low temperature on organism. This extensively studied and applied technique not only promotes the progress in biology, medicine and other sciences, but also will create huge benefits for agriculture, stockbreeding, pharmacy and food manufacturing. In particular, the cryobiology has engendered far-reaching influence on environment protection, to severe endangered animal salvation and biodiversity conservation. Laboratory animals are important experimental materials for life sciences. Several scientific trends have increased the large number of mouse models and placed an intolerable load on the financial and infrastructural resources needed to maintain these models as conventional breeding colonies in recent years. The cryobiology has been applied for breeding, preservation of genetic resources, and development of new strains of laboratory animals. Anyway, the preservation of mice model will establish the furthersome condition for further studies of mammalian genetics and difficult and complicated diseases.