Objective To design the barrier system of containing animal husbandry and animal experiment according to the principle of efficient, safety, efficiency and utility. Method The combined transportation unit, life unit, bufferl unit and experimental unit were mainly designed in this barrier instrument. During the process of the animal and goods transfer, there is a automatically controlled sterilizing system. Result The design improved work efficiency and guaranteed effectively cleaning degree of laboratory animal. Conclusion Results shows that the multiplex barrier system will be aviable not only for animal production but also for animal experiment in miniature laboratory animal facihies of SPF and clean rodents.