Objective To observe relationship between some serum index, such as T-BIL, ALT, AST, TP, ALB, and gender as well as age in C57BL/6J-HBV transgenic mice.The differences between HBV mice and normal C57BL/6J mice in blood serum also determined. Methods Eight-week, twenty-four-week C57BL/6J-HBV transgenic mice and normal C57BL/6J were put into experiments, T-BIL, ALT, AST,TP and ALB of blood serum were determined. Results There was significant difference of T- BIL,ALT,AST, TP and ALB between HBV mice and C57BL/6J mice. The great differences of ALT and AST value were also observed in different age HBV mice. Conclusion The value of T-BIL,ALT,AST,TP and ALB is remarkable higher in HBV mice than that in C57BL/6J.The value of ALT and AST is related to the age of HBV mice and unrelated to the gender.