STK15的表达对NIH3T3细胞的影响
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R73-3

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辽宁省科技计划项目?


Stimulating Effect of STK15 Gene on the Proliferation of NIH3T3 Fibroblasts
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    摘要:

    目的构建pcDNA3.1-STK15表达质粒,探讨STK15基因对小鼠成纤维细胞(NIH3T3)的影响。方法构建pcDNA3.1-STK15质粒,将其转染NIH3T3,应用RT-PCR、免疫细胞化学和Western印迹方法检测STK15的表达;MTT法检测细胞增殖能力;Transwell检测细胞侵袭能力。结果转染pcDNA3.1-STK15质粒的NIH3T3细胞在48 h有STK15的表达,而且该细胞的增殖速度和穿透Matrigel胶的细胞数均明显高于对照组(P〈0.05)。结论STK15基因具有增加细胞增殖和细胞侵袭力的功能,进而形成肿瘤。

    Abstract:

    Objective To investigate the possible effect of STK15 gene on the NIH3T3 fibroblasts.Methods\pcDNA3.1-STK15 expression plasmid was constructed and transient transfected into NIH3T3 cells.The effects of STK15 gene on the NIH3T3 fibroblasts was examined by RT-PCR,immunocytochemistry and Western blot,respectively.The cell proliferation rate was measured by MTT.The capability of cell invasion was observed by Transwell.Results STK15 expression was significantly upregulated in pcDNA3.1-STK15 plasmid-transfected N...

    Key words:STK15
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朱焱,王禄增,杨威,于洋,王维,董婉维,汪瑛,秦英,郑志红. STK15的表达对NIH3T3细胞的影响[J].中国比较医学杂志,2010,(2):17~20,I0002.

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