Objective The anti-tumor peptide of human endostatin was cloned into eukaryotic expression vector pVAX1 and its bioactivity was assayed.Methods\The nucleotide sequence encoding 1-30 amino acids(30-peptide,sequence RGIRGAD was changed to RGDRDG) of human endostatin was synthesized and inserted into plasmid pVAX1.The signal peptide coding sequences of collagen XVIII was also linked to the 5'-end of target gene.Then the recombinant plasmid was directly injected into the tumor tissue in mice.ELLISA,immunohistoc...