Objective To establish a rapid SNP genotype assay for the leptin~(ob) and leptinr~(db) alleles((single-)tube bi-directional allele specific amplification((SB-ASA),) and compared it with the traditional method of PCR-restriction endonuclease digestion.Methods\Seven and nine offsprings of ob/+ and db/m heterozygous mice,respectively,were genotyped by both the SB-ASA and PCR-restriction endonuclease digestion.Results All the offsprings were successfully genotyped by both the two methods,and the results were id...