ZHANG Sheng1,XIAO Gao-fang2,HUANG Li-zhen1,Nashunbayaer1,JIA Jun-shuang2,YAO Zhi-fang2,XIAO Dong1,GU Wei-wang1(1.Institute of Comparative Medicine & Center of Experimental Animals,2.Cancer Research Institute,Southern Medical University,Guangzhou 510515,China)
Objective To establish lentivirus-mediated in vitro gene delivery systems for porcine embryonic fibroblasts(PEFs) of Tibetan miniature pigs.Methods According to the standard protocol from Invitrogen,lentiviruses carrying EGFP gene were produced,followed by confirming that lentiviruses harboring EGFP gene were successfully made and PEFs were successfully infected through EGFP assay under fluorescent stereo microscope after infecting PEFs.Results PEFs of Tibetan miniature pigs were successfully and efficientl...