Two Spontaneous Type 2 Diabetic Mice and Their Difference of Phenotype
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Shanghai Institution of Material Medical，Chinese Academy of Science，Shanghai 201203，China
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摘要:
目 的 分 别 以 C57BL /6JSlac 和 C57BL /KsJ-db / + 表型正常小鼠为对照组，比 较 自 发 性 2 型 糖 尿 病KK-Ay /Ta 和 C57BL /KsJ-db / db 小鼠的体生长曲线、糖代谢曲线、血清胰岛素水平、主要脏器重量、脏器系数 等生物学特性的差异，并探讨其肾脏、肝脏和胰腺等组织病理学变化。方法 在各自实验周期内，每 2 周测定实验组和对照组小鼠的体重、血糖以及血清胰岛素水平，实验结束后处死，脏 器、脂 肪 称 重，部分组织制作病理切片。结 果(1)KK 小鼠体重远高于 db / db 小鼠，且同品系间雄性小鼠重于雌性小鼠(P < 0. 05) ;(2)同品系间雄性小鼠的血糖值明显大于雌性小鼠(P < 0. 05) ，db / db 小鼠出现血糖异常症状比 KK 小鼠早，且血糖值大 于 KK 小 鼠(P < 0. 05) ，而 KK 小鼠血糖异常持续时间则较 db / db 小鼠长;(3)KK 小鼠的血清胰岛素水平明显高于 db / db 小鼠(P < 0. 05) ，同品系雌雄小鼠间没有明显差异(P > 0. 05) ;(4) 雄 性 KK 小鼠脂肪系数及部分脏器萎缩程度大于雌性，而 db / db小鼠雌 雄 间 则 无 明 显 差 异 ( P > 0. 05 ) ，同 时 db / db 小鼠脾脏和胰腺的萎缩程度及脂肪系数大于 KK 小 鼠(P < 0. 05) ，而 KK 小鼠肝脏的萎缩程度则大于 db / db 小鼠;(5)糖尿病模型小鼠肾脏、肝脏以及胰腺组织均出现明显病变。结论 KK-Ay /Ta 和 C57BL /KsJ-db / db 小鼠均是肥胖的，伴有高血糖、高度胰岛素抵抗，肝脏、肾 脏 病 变 和胰岛功能不足的适用性 2 型糖尿病动物模型，且 db / db 小鼠血糖出现异常比 KK 小 鼠 早、脂 肪 系 数 大，而 KK 小 鼠血糖异常持续时间较 db / db 小鼠长，同时血清胰岛素水平远大于 db / db 小鼠。
Abstract:
Objective To investigate biological parameters and morphology in spontaneous type 2 diabetic KK-Ay /Ta and C57BL /KsJ-db / db mice. Methods In experiment the body weight，glucose and serum insulin were measured every two weeks. At the end of experiment，the mice were sacrificed for organ，fat weight and morphological study. Results① The weight of KK mice were higher than that of db / db mice，and the males were higher than the females in the samestrain(P < 0. 05) . ② The glucose of male mice were higher than the female，the abnormal glucose levels of db / db mice were detected earlier and higher than those of KK mice(P < 0. 05) . But duration of KK mice abnormal glucose was longer than db / db mice. ③ The serum insulin of KK mice were higher over db / db mice significantly( P < 0. 05) ，and they did not have obvious difference in the same strain(P > 0. 05) . ④ The fat to body weight ratios and atrophy of some viscera in male KK mice were more serious than the female，but there was no obvious difference in db / db mice. Atrophy of spleen and pancreas of db / db mice became more serious than that of KK，however，so did in liver of KK mice. The fat to body weight ratios of db / db mice was elevated than those of KK mice (P < 0. 05) . ⑤ The spleen，liver and pancreas of diabetic mice model had serious pathological deterioration. Conclusions The KK and db / db mice could be used as appropriate disease models of type 2 diabetes mellitus with hyperglycemia，hyperinsulinemia，obesity.