Objective To propagate SHIV1157ipd3N4 virus stock via passages in Chinese-origin monkey peripheral blood mononuclear cells ( PMBCs ). Methods The PBMCs from Chinese-origin rhesus macaque were cocultured with SHIV1157ipd3N4，a CCR5-tropic chimeric simian-human immunodeficiency virus (SHIV) strain. The level of P24 antigen in the supernatant was tested continuously. The virus stock was collected when the viral duplication reached a peak. The env gene of SHIV1157ipd3N4 was analyzed，and the viral load，P24 antigen level and TCID50 were determined. The rhesus monkey G1004V was infected with this batch of SHIV1157ipd3N4 intravenously. The viral load in plasma and the changes of CD4 + / CD8 + ratio were analyzed by real-time RT-PCR and flow cytometry. Results Totally 243 mL virus stock was propagated in monkeys PBMCs. The viral load was 1. 586 × 108 copies/mL，the level of P24 antigen was 1. 16 × 103 pg /mL and had 3. 16 ×103 50% tissue culture infective dose (TCID50 ) in 1 mL of cell-free SHIV1157ipd3N4. There was no variation in the gp120 sequence of env gene and this SHIV1157ipd3N4 stock was still exclusively CCR5-tropic. The monkey G1004V was infected and had a high level of viral loads in plasma. Conclusion This panel of pathogenic R5-tropic SHIV1-157ipd3N4 was adapted in Chinese-origin rhesus monkeys PBMCs，and is stable and suitable to serve as an animal model.