Objective To study the mechanism of the influenza virus infection in BALB / c mice with A / California /7 /2009Influenza H1N1 virus. Methods Sixty BALB / c mice were randomly divided into 2 groups:experimental group and control group，each group consisted of 30 mice. The CA7 influenza virus was used to establish influenza models.The expressions of lung IL-6，TNF-α，and IL-2 in experimental group of 5th day control group were detected by enzyme linked immunosorbent assay(ELISA) . Results The lung tissue IL-6，TNF-α levels were significantly higher，IL-2 levels were significantly lower than that in the control group，the differences were significant ( P < 0. 05 ) . Conclusions The cytokines IL-2，IL-6 and TNF-α may participate in the whole process of influenza A H1N1 virus infection and their lung levels are positively correlated with the severity of the disease.