Objective The ELISA kit for HIV-1 p24 is sometimes used for SIV p27 detection. The aim of this study was to eclarify whether there is any dependence and correlation between the results of detecting SIV p27 antigen by HIV-1 p24 ELISA kit and SIV p27 ELISA kit. Methods SIV p27 antigens were detected qualitatively and quantitatively by HIV-1 p24 ELISA kit and SIV p27 ELISA kit，respectively，and the results were compared by regression and correlation analysis. Results The sensitivity of detecting SIV p27 antigen was 150 pg /mL by HIV-1 p24 ELISA kit while 62. 5 pg /mL by SIV p27 ELISA kit. The qualitative results obtained by HIV-1 p24 kit consisted with those by SIV p27 kit on detecting SIV p27 antigen in virus stock and plasma. The statistical analysis of quantitative results indicated linear regression R2 = 0. 857，linear correlation r = 0. 926，P < 0. 01 in virus stock，while R2 = 0. 512，r = 0. 716，P < 0. 05 in plasma. The quantitative results turned out to be positive linear regression，which was higher in virus stock and lower in plasma. Conclusions HIV-1 p24 ELISA kit can be used for detecting SIV p27 antigen in virus stock and plasma qualitatively replacing SIV p27 ELISA kit，but quantitatively was appropriate in virus stock only.