Objective To establish infertile animal model through inflammation by directing infection mouse oviduct with staphylococcus aureus. Methods Directing inoculation concentration of 1 × 109 / mL of staphylococcus aureus into mice tube，making the model of chronic inflammation in the fallopian tube，observing the pathological inflammatory changes and pregnancy of mice. Results Seventy -five days later，mouse pregnancy rates and tubal patency rate in model group were significantly lower than control group(P < 0. 001) . In model group，the tubes had different degrees of dropsy，empyema and rigid tube had different levels of adhesion with surrounding tissue by visual observation. The fallopian tube lumen was completely obstructed by foreign granulation tissue and the whole layer was full of a large number of chronic inflammatory cell infiltrations by pathological observation. Conclusion That the concentration of 1 × 109 / mL of Staphylococcus aureus were inoculated could successfully establish a mouse model of salpingitis infertility.