Objective To explore establishment a stable model of rat chronic renal allograft rejection. Methods Inbred F344 and Lewis rats were used as the donors and the recipients. Used the left kidney as a donor kidney，dressing for the kidney in vivo and in situ perfusion. Epidural catheter within the renal vein as a temporary stent tube，end-to-end of the six acupuncture anastomosis. Artery end-to-side continuous suture; ureter and bladder with the bladder flap anastomosis，Used cyclosporine A 5 mg / kg·d to 30 days after operation to prevent acute rejection from three days before operation.Results The total time of operation about 120 ～ 180 min，success rate about 90. 9% (40P44) ; receptors can survive up to 60 d. the complications include anastomotic bleeding，venous thrombosis and acute rejection etc. Conclusion Dressing for kidney in vivo and in situ from renal perfusion is a simple and reliable method. Stent vein of end-to-end anastomosis，abdominal aortic end-to-side anastomosis can be achieved stable rate of success is worth promoting. Skilled surgical and vascular anastomosis operation is the key to success.