Objective To compare the adaptability of HET-CAM eye irritation test for products and ingredients.Methods Test 19 ingredients and 23 products by HET-CAM and Draize eye irritation protocol. Compare the data from in vitro and in vivo test. Result Compare in vitro data and in vivo data，The Kappa coefficient ，sensitivity，specificity are 0. 826，100% ，85. 75 for ingredient. 0. 531，81. 8% ，77. 8% for cosmetics as well. Conclusion HET-CAM eye irritation test can be used to be alternative test of Draize test. HET-CAM system is more suitable for ingredients test.