Objective To establish an animal of diet induced non-alcoholic fatty liver disease ( NAFLD) and hyperglycemia，and to investigate its features． Methods 64 rats were randomly divided into 2 groups． The control group ( fed with normal diet) 32，high glucose and high fat group ( fed with high sugar and high fat diet) 32，continuous feeding of 12 months． on the 3month、6months、9 months、12 month of the experiments，we observe animal weight，visceral fat weight; Comparing relevant in the blood lipid levels，blood sugar，inflammatory mediators aspects of biochemical indicators and observed histopathologically． Results Compared with the normal control group，weight and visceral fat weightof the animals of high glucose and high fat groups increased obviously; the level of ALT，FFA，LPS，TNF，FPG，FINS and HOMAIR of hig sucrose and high fat groups of every phases were higer，but the level of HOMA-beta of 6H group rats first showed the compensatory increase，then progressive decrease in sequence． The pathological and histological display liver produces serious steatosis，fatty liver and hepatitis，fibrosis and cirrhosis occurs; Over time progress islet gradually atrophic， accompanied by inflammatory infiltrate; Fat cells gradually increasing and accompanied by inflammatory infiltrate． Conclusion High sugar and fat diet can establish rats models of NAFLD and hyperglycemia，this model can be play important role in NAFLD and related diabetes research．