Objective To investigate the anesthesia management and effect of cardiopulmonary bypass ( CPB) on minipigs． Methods Thirty-four laboratory minipigs were divided into two groups: the cardiac arrest group ( 18 pigs) underwent tricuspid valve replacement with autologous pericardium under cardiopulmonary bypass，and the parallel group ( 16 pigs) receiving the tricuspid valve replacement with autologous pericardium and CPB in parallel． The dosages of anesthetics and vasoactive drugs，maintenance times of basic anesthesia and general anesthesia，recovery time after anesthesia，survival rates at 3 days and 1 week and hemodynamics of each period were recorded，and the effects of basic anesthesia and general anesthesia were evaluated． Results All the 34 minipigs underwent surgery under general anesthesia smoothly with stable hemodynamics in each period，with only one pig died 3 days after the surgery． The survival rate was 97． 1% ． The anesthesia effect was satisfactory． Conclusions Rational and combined adoption of anesthetics and vasoactive drugs，careful observation and swift and right treatments are key points of successful cardiac surgery anesthesia under cardiopulmonary bypass on minipigs．