Objective Methods cells from the bone marrow，spleen and thymus were analyzed by flow cytometry． HSCs were sorted and cultured in vitro． Results Compared with wild type mice，there are no great differences in the cell percentage and function of bone marrow，spleen and thymus in Cramp over expression mice． Conclusions Compared with the wild type mice，there was no great difference in the HSCs ` differentiation and single cell clone in Cramp over expression transgenic mice．