Objective Methods MSCs were isolated from mice limb bone chips and in vitro culture． The proliferation and growth characteristics of MSC were observed during primary and passage cultures． Results Cells culture showed that mice bone chips derived MSCs exhibited the characteristics of mesenchymal cells． MSCs clones assay of WT mice were higher than Terc^－/－mice in primary and passage cultures ( P＜0. 01 ) ． Conclusions MSCs derived from mice bone chips can become an easy and reproducible method that does not require depletion of hematopoietic cells by sorting or immunomagnetic techniques ． MSCs of short telomere mice( Terc^－/－mice) has a more feeble feasibility in cells proliferative ability than WT mice as the seed cells for tissue engineering reconstruction．