The Effects of Pioglitazone on Expression of Protein Tyrosine Phosphatase 1B in Muscle of db /db Mice
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Department of Endocrinology，Nanjing General Hospital of Nanjing Military Region，Nanjing 210002，China
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摘要:
目的 探讨吡格列酮对 db / db 小鼠骨骼肌蛋白酪氨酸磷酸酶 1B( protein tyrosine phosphatase 1B，PTP1B) 表达水平的影响。 方法 将 20 只 4 周龄 db / db 小鼠随机分为两组( 吡格列酮组和 db / db 对照组) ，每组 10只，分别给予吡格列酮 10mg / kg·d 和安慰剂灌胃。另设 10 只同周龄 db /m 小鼠，给予安慰剂灌胃作为非糖尿病对照( db /m 对照组) 。每周监测体重、血糖，4 周后用蛋白印迹法检测各组小鼠骨骼肌组织中 PTP1B 蛋白含量。 结果db / db 组小鼠骨骼肌 PTP1B 表达显著高于 db /m 组，给予吡 格 列 酮 干 预，血 糖、胰岛素抵抗指数显著低于 db / db 组( P＜0. 05) ，骨骼肌 PTP1B 表达水平亦显著降低( P＜0. 05) 。 结论 吡格列酮改善胰岛素抵抗，可能与降低骨骼肌PTP1B 蛋白表达有关。
Abstract:
Objective Methods Twenty db / db mice aged 4 weeks were randomized to pioglitazone group ( PIO group) and db / db group，fed with 10mg / kg·d pioglitazone and placebo respectively． Ten age-matched db /misty mice were concurrently treated with placebo as non-diabetic control group ( db /m group) ． Body weight and blood glucose were measured every week． After 4 weeks' treatment，the expression of PTP1B in muscle of mice were determined with Western blotting． Results The expression of PTP1B in muscle of db / db mice significantly increased compared with db /m( P＜0. 05) ． Pioglitazone treatment significantly decreased fasting blood glucose and HOMA-IR． The expression of PTP1B in muscle of pioglitazone-treated mice obviously decreased compared with db / db group ( P＜0. 05 ) ． Conclusions Pioglitazone treatment could improve insulin resistance possibly by reducing the expression level of PTP1B in the muscle．
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卢 斌,顾 萍,邵加庆,杜 宏,王 坚.吡格列酮对 db /db 小鼠骨骼肌蛋白酪氨酸磷酸酶 1B 表达的影响[J].中国比较医学杂志,2012,22(01):48~50.