Objective Methods Mouse MIP-1α and B7-1 genes were synthesizeed and amplification by PCR．Target genes were directly connected with Lentivirus vector，the production of which were transformed into Bacterium coli DH5α cells，and the positive colones were identified by PCR and direct sequencing analysis． Then the plasmids of MIP-1α and B7-1 genes infected 293T cells，respectively，green fluorescence protein ( GFP ) in 293T cells was observed by fluorescence microscope; Western Blot was used to test protein expression of MIP-1α and B7-1 genes and Real-time PCR was used to detect the titer of lentivirus． Results Lentivirus-mediated mouse MIP-1α and B7-1 gene vectors were successfully constructed and the titer of which was 2. 00E + 8 TU /ml tested by real-time PCR． Conclusions Lentivirusmediated mouse MIP-1α and B7-1 gene vectors were successfully constructed and lay a foundation for gene therapy with lymphoma in the future．