壮阳药效动物实验评价方法的改进
作者:
作者单位:

1. 南方医科大学新药评价中心,广州 510515; 2. 南方医科大学实验动物中心,广州 510515

作者简介:

通讯作者:

中图分类号:

R-332

基金项目:


Seek the More Effective Screening Method on the Assessment Methods of Strengthening Yang Efficacy
Author:
Affiliation:

1. Center of Drug Evaluation and Research,Southern Medical University,Guangzhou 510515,China;2. Laboratory Animal Center,Southern Medical University. Guangzhou 510515,China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 对壮阳药效评价方法进行优化,以探求更为有效的候选药物筛选方法。 方法 模型组大鼠均灌胃给予西地那非 10 mg / kg,对照组大鼠给予相同容量的生理盐水灌胃,每天 1 次,连续灌胃给药 14 d。每次给药后连续记录 2 h 内大鼠阴茎勃起、阴部理毛、爬背次数和参与动物数,并计算 PEI 值。采用改良的壮阳药效评价方法,整个过程采用摄像头观察并在不同时间段给予雌鼠诱导。 结果 在雌鼠诱导组中,模型组大鼠给药后 2 h PEI值较对照组明显升高( P<0. 05) 。在模型组中,与人为观察组相比,摄像头观察组大鼠给药后 2 h PEI 值明显升高( P<0. 05) ; 与非雌鼠诱导组相比,雌鼠诱导组大鼠给药后 2 h PEI 值 明 显 升 高( P<0. 05) ; 与持续诱导组相比,间断 诱导组 2h PEI 值无明显差异,但 60 ~ 120 min 时间段 PEI 值明显升高( P<0. 05) 。 结论 优化后的壮阳药效评价方法能更为有效的评价候选药物的壮阳药效。

    Abstract:

    Objective Methods Rats of model groups were given silaenafil 10mg / kg by gavage. Rats of control groups were given normal saline only. All rats were gavaged once a day for two weeks. Rats were observed for 2 hours after each drug administration for penile erection,genital grooming and possible copulation mounting. The number of responding rats was recorded along with the number of sexual activity episodes ( penile erection,genital grooming or copulation mounting) . Penile erection index ( PEI) was calculated for each group by multiplying the percentage of active rats ( responding rats) by the total number of activity episodes. By using the modified assessment method,male rats were observed by camera. Meamwhile,we also stimulated the male rats by female rats at different periods. Results Among female rat-stimulated groups,the model groups compared with the control groups,PEI was significantly increased ( P<0. 05) . Among the model groups,the camera observed group compared with the human observed group, PEI was significantly increased ( P<0. 05 ) . Compared with non-female rat-stimulated group,PEI was significantly increased in female rat-stimulated group( P<0. 05) . The 2 h PEI showns no significant differences between sustained induction group and intermittent group. But,during the period of the second hour,PEI was significantly increased in intermittent group ( P<0. 05 ) . Conclusions After optimized,the assessment methods can evaluated the strengthening yang efficacy of candidate drugs more effective.

    参考文献
    相似文献
    引证文献
引用本文

曹 莹,孔焕育,丘玉昌,王杞妹,崔东方,林继红.壮阳药效动物实验评价方法的改进[J].中国比较医学杂志,2012,22(01):66~68.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-10-27
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2025-07-09
  • 出版日期: