十一五新药专项支持(2008ZX09305-001)。［通讯作者］ 张晓娟,E-mail:zhangmice@hotmail.com。
GE Wen-ping
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, ChinaZHANG Xu
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, ChinaGAO Xiang
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, ChinaLIU Ning
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, ChinaZHANG Hai-tao
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, ChinaZHANG Xiao-juan
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College, Beijing 100021, China目的按现行国标《实验动物微生物学检测方法》和《实验动物寄生虫学检测方法》对我国商品化的无特殊病原体(specific pathogen free,SPF)小鼠进行微生物状况检测,为生产高质量的实验动物提供依据。方法对五家主要实验动物生产单位生产的ICR、KM、C57BL/6、BALB/c及BALB/c-nu 品系的SPF级小鼠进行随机抽检。检测抽检小鼠所携带的微生物和寄生虫情况。结果在抽检的SPF级小鼠中,病毒污染主要包括呼肠孤病毒Ⅲ型(Reo-3)、小鼠肺炎病毒(PVM)和多瘤病毒(POLY)； 主要细菌污染为肺炎克雷伯杆菌、金黄色葡萄球菌和绿脓杆菌。这些微生物病原体中,除条件性致病菌绿脓杆菌外,其他病原体对小鼠本身和实验研究均具有一定的影响。
ObjectiveTo provide reference for producing a good laboratory animal production and management, we randomly analyzed microbes and parasites of Specific pathogen free(SPF)mice commonly used in China.Methods The current national standards《Laboratory animal—microbiological standards and monitoring》and《Laboratory animal—parasitic standards and monitoring》were used to tested the microbes and parasites in the samples of SPF mice strains commenly used in China, including ICR,KM,C57BL,BALB/c and BALB/c-nu.ResultsThe results showed that the main virus carried by SPF mice were Reo-3, PVM and POLY, the bacteria contamination included klebsiella pneumoniae, Staphylococcus aureus and Peudomonas aeruginosa. They all affect the mice and experiment in certain degree except for Peudomonas aeruginosa.
葛文平,张旭,高翔,刘宁,张海涛,张晓娟.我国商业化SPF级小鼠病原体污染分析[J].中国比较医学杂志,2012,(3):65~68.
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