Objective To observe the morphology change of human umbilical cord mesenchymal stem cell (hUC-MSC) cultured on polypyrrole (PPy) under the conditions of different styles of electric stimuli. MethodhUC-MSC were identified by flow cytometry and inverted phase contrast microscope. Passage 3 to 6 hUC-MSC were co-cultured with PPy. Current-controlled and voltage-controlled electric stimuli (ES) were applied to the cells. Cells cultured in αMEM with 10% fetal bovine serum and cells cultured on PPy without ES were used as control. Scan electron microscope (SEM) and inverted phase contrast microscope were used to observe the morphology change of hUC-MSC. ResultThe effect of ES on the adhesion and morphology change of hUC-MSC was evident and similar in different groups. Conclusion ES has distinct effect on hUC-MSC adhesion and morphology and can be used as a method of cell regulation. More research are needed.
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