Objective In order to prepare immunoglobulin G (IgG) secondary antibody of non-human primates such as Macaca fascicularis and Macaca mulatta and establish a quick serological method to diagnose their diseases. Methods Macaca fascicularis IgG was isolated and purified from Macaca fascicularis serum by precipitated use saturated ammonium sulfate and affinity chromatography technology with Agarose-Protein G. After identified by SDS-PAGE electrophoresis murine monoclonal antibody(McAb)were produced by conventional immune method and hybridoma technology.Monoclonal antibody was produced when C57BL/6 mice was immunized three times and its spleen cells were fused with SP2/0-Ag14 myeloma cells. Indirect ELISA and Western blot methods were used for identification. Result5 strains of myeloma cells acquired which were named 2B₆, 2B₇, 3B₂, 5E₄, 2D₉.The antibodies they secret can all react well with both IgG and their serum of Macaca fascicularis and Macaca mulatta. There are no cross reaction with other species such as east-northern tigers and dogs. Conclusion The 5 strains of monoclonal antibodies can secrete antibody chronically and steadily. The study can be valuable in establishing a quick serological method in diagnosing the infectious disease.