ObjectiveTo make a reproducible diabetes mellitus model in macaques by injecting streptozotocin (STZ). Methodswe used STZ (30 mg/kg of body weight, intravenously, 3days) to five macaques. The dynamic changes of the glucose, C-peptide and insulin contents in plasma were measured for 12 months. The IVGTT were made at 2 and 3 month. ResultsThree of five monkeys became markedly hyperglycemic, 2 other monkeys received a second and third dose of STZ intravenously (30 mg/kg) after the first dose. Compared with the contents before injection,the glucose contents increased significantly while C-peptide and insulin contents reduced in plasma in macaques 1 week after streptozotocin injection(P<0.01),Diabetic monkeys also had abnormal responses to an intravenous glucose tolerance test at 2 and 3month. ConclusionThe results showed that low dose with 30 mg/kg STZ could lead to the significant changes in glucose, C-peptide and insulin content stably, indicating that macaques diabetes mellitus model was established successfully．