江丽萍(1965-),女,主要研究方向:药物研究及教学教育研究工作。E-mail:JLP653@sina.com.cn。
上海市科技发展基金项目(071409010)。
目的探讨玉米蛋白及其水解产物对自发性高血压大鼠(SHR)血压的影响。方法6~8周龄雄性SHR大鼠60只随机分为A组(玉米蛋白组)、B组(胃蛋白酶水解产物组)、C组(胰蛋白酶水解产物组)、D组(复合酶水解产物组)和E组(生理盐水对照组),每组12只动物,均以1 g/kg体重剂量灌胃,测定灌胃0、1、2、4、6、8h后SHR血压值。结果胃蛋白酶水解产物、胰蛋白酶水解产物和复合酶水解产物在0~6 h内对SHR的血压升高具有明显抑制作用,其中0~2 h内降血压效果明显；各组水解产物中,以复合酶水解产物在0~6 h内降压效果最佳,SBP与生理盐水对照组、胃蛋白酶水解产物组和胰蛋白酶水解产物组相比具有极显著差异(P＜ 0.01)。 结论玉米蛋白胃蛋白酶、胰蛋白酶和复合酶水解产物能有效地降低SHR大鼠的血压,为其在保健食品领域及医药方面的开发应用提供了参考。
Objective To study the antihypertensive effects of s corn protein and its hydrolysates on spontaneously hypertensive rats (SHR)．MethodsSixty 6~8 week-old male SHR were randomly divided into 5 groups:group A (corn protein), group B (corn protein''s hydrolysate prepared with pepsin), group C(corn protein''s hydrolysate prepared with trypsin), group D (corn protein''s hydrolysate prepared with complex enzyme) and group E(saline). Each group were orally adiministered at dose of 1g/kg body weight. Subsequently, systolic blood pressure of SHR was measured at a fixed time interval (0,1, 2,4, 6 and 8 h) post-adiministration. ResultsResults showed that all hydrolysates had significant inhibition on blood pressure of SHR and compound enzyme hydrolysate had the strongest effect on antihypertension during 0~6 h, with SBP decreased significantly which compared with group A, B, C and E (P<0.01). ConclusionA significant decrease in systolic blood pressure of SHR was observed in group, B, C and D.
江丽萍,余琛琳,汤球,蔡丽萍,崔淑芳.玉米蛋白及其水解产物对自发性高血压大鼠降血压作用的研究[J].中国比较医学杂志,2012,(7):9~12.
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