ObjectiveTo explore the effect of ginsenoside Rb3 on chromosome aberration in Chinese hamster lung (CHL) cells. MethodsTo determine the IC50 of ginsenoside Rb3 in CHL cells, and to establish the range of doses according to the IC50 and induction of chromosome aberration. The results were judged according to the positive response standard. ResultsNegative response was found at 6 h and 24 h after the treatment with ginsenoside Rb3 and at 6 h after the addition of S9 mixture. ConclusionsUnder the conditions used in this study, ginsenoside Rb3 does not induce chromosome aberration in CHL cells in vitro.