徐艺玫(1973-),女,副研究员,研究方向:动物实验与管理。E-mail:xymeimei@163.com。
国家“十一五”科技支撑计划重点项目(2009BAI83B02)。
目的建立一种简单快速观察腹腔内接种包虫病动物模型的方法。方法按2000个原头节/mL剂量,用多房棘球蚴对灰仓鼠进行腹腔接种,采用肉眼观察法和显微镜镜检法在第10天,15天,18天,22天,39天和60天观察灰仓鼠腹腔内包囊、包囊液和原头节的生长情况；设空白对照组10只。结果通过肉眼观察和显微镜镜检,发现灰仓鼠在接种后的第18天有原头蚴生长；第15天,18天和22天包囊增大、增多；第39天有成熟原头节胚基生长；第60天有不同发育程度的原头蚴。随着接种时间的延长,包囊重量与传代时间成正比。结论本试验为制备包虫病动物模型提供了简单快速的观察方法。
ObjectiveThis study was designed to establish a simple and rapid methods for observing animal models of echinococcosis.MethodsMultilocular echinococcus in a dose of 2000 protoscolex/mL were intraperitoneally inoculated in the C. migratorus. Using a simple visual observation and microscopic examination method, the growth of intraperioneal cyst, cyst fluid and protoscoleces was observed in the 10d,15d, 18d, 22d, 39d and 60d after inoculation. ResultsProtoscoles were found in the 18d, and cysts became big and many in the 15d,18d and 22d. There was protoscoles blastemal growth in the 39d. Different degree of protoscolex was developed in the 60d. With the extension of the inoculation time, the cyst weight increased. Conclusion The methods for examination of animal models of echinococcosis were established.
徐艺玫,刘斌,史深,燕顺生,廖力夫.镜检法在腹腔内接种包虫病动物模型中的应用[J].中国比较医学杂志,2012,(9):58~62.
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