Objective To investigate whether seasonal vaccine induces anti-virus activity against H7N9 and cross-reactive immune response against related H1/H3/H5subtype influenza viruses.Methods A/Anhui/1 (H7N9) were pre-incubated with sera vaccinated with seasonal vaccine and A/California/07/2009 (H1N1), A/PR/8/34 (H1N1), A/Brisbane/59/2007 (H1N1), A/Brisbane/10/2007 (H3N2), A/Shenzhen/406H/2006 (H5N1), A/Vietnam/1203/2004 (H5N1) were pre incubated with ferret sera infect with H7N9 through Micro-neutralization and HI Assay. Results Human sera from the donors were tested for HI and neutralization activity against influenza (H7N9) preparationsremained at undetectable levels after seasonal vaccination, and ferret sera were tested for HI and neutralization activity against H1/H3/H5 type influenza viruses either.Conclusion The neutralizing antibodies raised in all donors sera vaccinated with seasonal vaccine cannot neutralize H7N9 influenza virus, and cannot cross-react to H1/H3/H5 subtype.