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首页 > 过刊浏览>2014年第24卷第1期 >62-67. DOI:10.3969.j.issn.1671.7856.2014.001.015
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血液免疫学指标在过敏性紫癜大鼠和兔模型及患儿体内的比较
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Comparison of blood immunological indexes in henoch-schonlein purpura rat and rabbit models and pediatric patients
Author:
  • LI Yan-hong

    LI Yan-hong

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • HUANG Lan

    HUANG Lan

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • ZHU Hua

    ZHU Hua

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • XU Yan-feng

    XU Yan-feng

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • SUI Xiao-long

    SUI Xiao-long

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • XU Yu-huan

    XU Yu-huan

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • HAN Yun-lin

    HAN Yun-lin

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • FENG Xiao-chun

    FENG Xiao-chun

    Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021, China
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  • QIN Chuan

    QIN Chuan

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China
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  • 摘要
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  • 参考文献 [27]
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    摘要:

    目的 通过检测过敏性紫癜大鼠及兔模型的血液学指标的改变，并与人类患者血液学指标改变进行比较，为进一步探索该病的发病机制、明确诊断及治疗提供帮助。方法 首先构建模型；进行过敏性紫癜大鼠及兔的症状观察和病理分析；血常规检测；检测大鼠、兔及患儿血清中细胞因子IL-2、IL-4、TNF-α，白细胞表面标记分子CD3、CD4、CD8等，免疫球蛋白IgA，IgE，IgG及补体C3，C4的含量。结果 模型大鼠和兔皮肤均有不同程度的出血斑点，皮肤，胃肠道，关节及肾脏也表现为不同程度的炎症反应；模型大鼠、兔及患者急性期血常规均有不同程度的白细胞（WBC）升高，淋巴细胞（LYM）及百分比（LYM%）下降，中性粒细胞（UEN）及百分比（UEN%）升高，血小板（TLP）正常；血清CD4+T细胞减少，CD4/CD8比值下降，IL-4及TNF-α水平升高；IgA升高，C3、C4减少；差别有统计学意义。但这些指标改变的程度不同，血清IL-2、IgG、IgE水平改变不完全一致。结论 过敏性紫癜大鼠及兔模型大部分血液学指标改变与人类相似，可为深入研究该病的发病机制、明确诊断及治疗提供帮助。

    Abstract:

    Objective To offer the help for exploring the pathogenesis, diagnosis and therapy of anaphylactoid purpura through detection of blood immunological indexes in rat and rabbit models, and comparation with changes of blood indexes in pediatric patients.Methods Establishment of rat and rabbit models; For anaphylactoid purpura, then take symptom observation and histopathological analysis; Blood routine tests; Detection of serum cytokines IL-2, IL-4, TNF-α; white cells surface markers CD3, CD4, CD8; immunoglobulins IgA, IgG, IgE, and complements C3 and C4 contents in rat, rabbit and pediatric patients. Results Rats and rabbits models manifested different degrees of skin Petechiae, and gastrointestinal, joint and kidney also showed various degrees of inflammatory reaction; Compared with the control group, rats and rabbits of model group and patients presented increased number of white blood cells (WBC), neutrophils (NEU) and percentage (NEU%), lymphocytes (LYM) and percentage (LYM%) decreased, normal count of (platelet) TLP at acute phase; serum CD4+ T lymphocytes was decreased, CD4+/CD8+ ratio were decreased, and TNF-α, IL-4 increased; serum immunoglobulin IgA level elevated, C3,C4 decreased, all had statistically significant differences between the two groups. But these indexes changed in different degrees, serum IL-2, IgG, IgE changes were not the same. Conclusions Hematological changes of Henoch-Schonlein purpura in rat and rabbit models were similar to humans. These results will provide a valuable basis for clarifying pathogenesis, diagnosis and treatment of HSP.

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李彦红,黄澜,朱华,徐艳峰,隋小龙,徐玉环,韩云林,冯晓纯,秦川.血液免疫学指标在过敏性紫癜大鼠和兔模型及患儿体内的比较[J].中国比较医学杂志,2014,24(1):62~67.

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  • 最后修改日期:2013-12-09
  • 在线发布日期: 2014-01-23
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