鼠抗人hMIC-l单克隆抗体抑制裸鼠移植人胰腺癌体内研究
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国家高技术研究发展计划‘863’资助项目(2007AA027485)。


Effective of anticancer mouse monoclonal antibody against hMIC-1 targets pancreatic tumor for nude mice in vivo
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    摘要:

    目的 初步研究鼠抗人hMIC-l单克隆抗体对胰腺癌体内给药的抗肿瘤活性,为hMIC-l抗体应用于肿瘤治疗提供实验依据。方法 2种人胰腺癌细胞株PANC-1和SW1990各腋窝皮下接种24只Balb/c裸鼠,共计48只皮下接种荷瘤裸鼠分别随机共分为8组,每组6只荷瘤鼠。模型对照组荷瘤裸鼠腹腔注射0.9%氯化钠注射液(10 mL/kg,NS,Biw,ip×8),阳性对照组荷瘤裸鼠腹腔注射键择(50 mg/kg,qw,ip×4),鼠抗人hMIC-l单克隆抗体组分别荷瘤鼠尾静脉内注射鼠抗人hMIC-l单克隆抗体(10 mg/kg,Biw,ip×8)共4周或(2 mg/kg,Biw,ip×8)共4周。观察荷瘤裸鼠日常表现、肿瘤生长、实验后肿瘤组织切片HE染色后光镜下的组织形态学改变。结果 荷瘤裸鼠尾静脉内注射鼠抗人hMIC-l单克隆抗体组裸鼠(10 mg/kg,Biw,iv×8)肿瘤生长缓慢,瘤体明显小于模型对照组,并呈现量效关系。镜下观察鼠抗人hMIC-l单克隆抗体组实验后肿瘤组织切片胰腺结构破坏,有大量淋巴细胞浸润,肿瘤细胞明显坏死,细胞溶解。结论 尾静脉内注射鼠抗人hMIC-l单克隆抗体(10 mg/kg,Biw,iv×8)能有效抑制裸鼠移植人胰腺癌PANC-1肿瘤生长,使瘤组织坏死、结构破坏。

    Abstract:

    Objective To investigate a antitumor effects of mouse original monoclonal antibody against hMIC-1 as intravenous administration with human pancreatic tumor in vivo and providing experimental data.Methods The fourty-eight mice were randomized into eight groups for loaded with two pancreatic tumor cell lines panc-1 or sw1990 respectively, and individual tumor growth was observed, antitumor efficacy was evaluated after using mouse original monoclonal antibody against hMIC-1 by intravenous administration.The pathological change with formalin fixed, paraffin embedded tissues section was viewed.Results There was a significant difference in tumor volume and weigt in intravenous injection of mouse original monoclonal antibody against hMIC-1 on load pancreatic tumor with nude mice group compared with that in the control group after four week treatment, and the mouse original monoclonal antibody against hMIC-1 demonstrated a close association between inhibition of tumor volume growth and dose-effective in the two xenograft models examined. Under examined microscope, the pancreatic tumor tissue was destroyed evidently in mouse original monoclonal antibody against hMIC-1 group.Conclusions The antitumor effect of intravenous injection for mouse original monoclonal antibody against hMIC-1 is better than that of systemic using gemcitabine.

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刘朝阳,王小兵,张伟.鼠抗人hMIC-l单克隆抗体抑制裸鼠移植人胰腺癌体内研究[J].中国比较医学杂志,2014,24(3):14~19.

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  • 最后修改日期:2013-12-30
  • 在线发布日期: 2014-04-08
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