Objective To measure the organ weights, blood physiological and biochemical parameters, and immune cells of BALB/cA-nu mice. Methods BALB/cA-nu mice at five and ten weeks of age were selected, and the organ weights, blood physiological and biochemical parameters were observed. The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were detected by FCM in BALB/cA-nu mice at six weeks of age in terms of its T, B lymphocyte cells and NK cell activity. Results At the same age, the weights of the body, liver, and kidney in male mice are significantly higher than females. The HGB、MCH、HCT in male mice are significantly higher than females(P< 0.05). The weights of the lung, liver, and kidney,and the parameters of TP、GLOB、CHO、TBIL、UN are significantly lower at five weeks of age comparing with ten weeks(P< 0.05). The BALB/cA-nu mice lacks T cells(0.18±0.06)%、CD4+T cells(0.26±0.08)%、CD8+T cells(0.13±0.04)%、The percentage of B cells is CD19+B cells(30.10±2.74)% and B220+B cells(30.55±2.77)%. The percentage of NK cells is(1.35±0.29)%, and the percentage of granulocytes is(47.90±5.48)%. The BALB/cA-nu mice lack T cells and the group of cells. Conclusion The study suggests that the organ weights, blood physiological and biochemical parameters are affected by age and gender in BALB/cA-nu mice that lack T cells immune function. The studied parameters of BALB/cA-nu mice are similar with the same strain in other countries.