The present situation, efficacy, problem and strategy of laboratory animal license management in Guangdong Province were reviewed in this article. The laboratory animal license management has been implemented for 10 years and showed good results. Now there are comprehensive management of quality, facilities, personnel, SOP, and unified item bank, and making standard of laboratory animals in Guangdong Province. The legislation of Guangdong laboratory animal management regulations is completed. Guangdong laboratory animal license is increased year by year. The distribution of industry and area is expanded. Through analysis of problems and search for strategy, Guangdong laboratory animal management will be further developed.