国家质检总局科研项目（2012IK194）。
目的 组合两种体外替代方法评价化妆品的眼刺激。方法 分别采用鸡胚尿囊膜血管试验（CAMVA）、牛角膜浑浊通透试验（BCOP）和Draize兔眼试验对60种化妆品进行测试，并预测眼刺激分类。结果 采用CAMVA法可以区分41种无刺激性样品和18种有刺激性样品；采用BCOP法可预测35种无刺激性、21种轻-中刺激性和4种严重刺激性样品；组合CAMVA-BCOP方法可明显提高刺激程度的区分能力，与体内实验分类一致性达98.3%。结论 组合CAMVA-BCOP的整合试验策略可用于化妆品的眼刺激评价，其预测范围可覆盖无刺激性到严重刺激性。
Objective To explore the use of integrated two methods in vitro in prediction of eye irritation caused by cosmetics.Methods Chorioallantoic membrane vascular assay (CAMVA), bovine corneal opacity and permeability (BCOP) and Draize rabbit eye irritation test were used to determine the predictive potential of eye irritation of 60 kinds of cosmetics. Results CAMVA method was able to distinguish 41 non-irritant samples and 18 irritant samples. BCOP method was able to predict 35 non-irritant samples, 21 mild-moderate irritant samples and 4 severe irritant samples. Combination of CAMVA and BCOP methods could obviously improve the identification ability of irritation, and the classification consistency with Draize rabbit eye irritation testing reached 98.3%. Conclusions The integrated test strategy combined BCOP with CAMVA can be used to appropriately predict ocular irritation of cosmetics, with a prediction range covering non-irritant to severe irritant samples.
秦瑶,程树军,黄健聪,黄玲,陈彧.整合CAMVA和BCOP方法检测化妆品的眼刺激性[J].中国比较医学杂志,2014,24(6):78~82.
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