Objective To explore the use of integrated two methods in vitro in prediction of eye irritation caused by cosmetics.Methods Chorioallantoic membrane vascular assay (CAMVA), bovine corneal opacity and permeability (BCOP) and Draize rabbit eye irritation test were used to determine the predictive potential of eye irritation of 60 kinds of cosmetics. Results CAMVA method was able to distinguish 41 non-irritant samples and 18 irritant samples. BCOP method was able to predict 35 non-irritant samples, 21 mild-moderate irritant samples and 4 severe irritant samples. Combination of CAMVA and BCOP methods could obviously improve the identification ability of irritation, and the classification consistency with Draize rabbit eye irritation testing reached 98.3%. Conclusions The integrated test strategy combined BCOP with CAMVA can be used to appropriately predict ocular irritation of cosmetics, with a prediction range covering non-irritant to severe irritant samples.