国家自然科学基金项目(31172276;31272514)。
目的 鉴定猴B病毒囊膜蛋白gD的抗原表位。 方法 利用生物信息学分析猴B病毒囊膜蛋白gD的二级结构、亲水性、表面可及性、抗原性指数以及柔韧性,得到可能的表位肽段;再利用合成肽与B病毒阳性血清进行ELISA验证,评价表位肽段的特异性和敏感性。 结果 在gD蛋白上预测得到7个表位肽段;ELISA测定结果显示,4个肽段(序列分别为46LPPLEQKTD54、106RGAPEATRSDA116、291PELAPEERGTSRTPGD306和361AVYLVRRRGR370)可与B病毒阳性血清发生免疫学反应,敏感性在40%~70%之间。结论 B病毒gD蛋白上至少存在4个线性化表位。
Objective To Identify B-cell epitopes on monkey B virus envelope protein gD. Methods Base on bioinformatics software, secondary structure, hydrophilicity, surface accessibility, antigenic index and flexibility of monkey B virus envelope protein gD was analysed and some potential peptide epitopes were forecasted. Then, the interactions between synthetic peptides and BV positive sera were detected by Enzyme-linked immunosorbent assay (ELISA). At last, The sensitivity and specificity of synthetic peptides was evaluated used 20 samples of Standard sera by ELISA. Result Seven epitopes were forecasted by Bioinformatics analysis. Four synthetic peptides, sequence as 46LPPLEQKTD54, 106RGAPEATRSDA116, 291PELAPEERGTSRTPGD306 and 361AVYLVRRRGR370could be reacted with positive sera pool. The sensitivity of 4 synthetic peptides changed form 40% to 70% and the specificity were 100% for 4 synthetic peptides. Conclusion There are at least four linear epitope on B virus gD protein.
叶华虎,袁菊芳,刘欢,范玉筠.猴B病毒囊膜蛋白gD的B细胞表位预测及鉴定[J].中国比较医学杂志,2014,24(11):20~26.
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