国家自然科学基金(81402625);国家973项目(2011CBA01006);广东省自然科学基金(S2013010014720);中国博士后科学基金(2014M550439);国际科技合作专项(2011DFA33290).
目的 对西藏小型猪GH基因(部分序列)进行SNP分析,筛选体型较小的基因型. 方法 采用PCR 产物直接测序法对108头西藏小型猪GH 基因5'端部分片段进行单核苷酸多态性(SNP)分析. 结果 通过比对发现GH基因共有5个变异位点,其中T45C位点的多态信息含量最高.不同基因型生长性状比较的结果显示:在GH基因中T45C突变位点上TC基因型6～8月龄的西藏小型猪的腹围值较小,G84A突变位点上AA基因型3～5月龄的西藏小型猪的体重、体长值较小,G93A 突变位点上GG基因型6～8月龄的西藏小型猪的体长、体高值较小.结论 在GH基因中T45C、G84A、G93A位点突变的TC、AA、GG基因型可能与体型较小有关.同时发现西藏小型猪在以上位点遗传变异程度大,具有较高的杂合度和遗传多样性,可以为选育工作提供比较丰富的素材.
Objective To screen genotypes of small body in Tibet minipigs by single nucleotide polymorphism analysis (SNP) of growth hormone (GH) gene. Methods The PCR products are sequenced. According to the results, SNP analysis are applied in GH gene 5' fragment of 108 Tibet minipigs. Results Five mutation points were found by comparison. The polymorphism information content of the T45C locus is highest. The results of growth traits of different genotypes showed that the abdomen of 6～8 month old Tibet minipigs of TC genotype at T45C mutation site is small; the weight and height of 3～5 month old Tibet minipigs of AA genotype at G84A mutation site is small; the body length and height of 6～8month old Tibet minipigs of GG genotype at G93A mutation site is small. Conclusion The AA TC, GG genotypes of T45C, G84A, G93A mutation sites in GH gene may be associated with small body. We also found that genetic mutations always occur in the above sites with high heterozygosity and genetic diversity, which can provide rich material for breeding research.
王玉珏,岳敏,顾阳,顾为望,田雨光.西藏小型猪GH基因部分序列的SNP分析[J].中国比较医学杂志,2015,25(1):50~54.
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