Objective To collect more than 200 kinds of species totaling more than 26,000 existing laboratory animals data worldwide to establish a largest and most comprehensive database of laboratory animal strains, based on the existing laboratory animal strain databases and related publications or references. Methods Based on MySQL database software to set up medium sized databases, and build search interface, input interface and database interface. Results In this work, a laboratory animal strain database was established, including data of 21596 mouse strains, 2062 rat strains, 13 monkey strains, 2 hamster strains, 5 dog strains, and 5 rabbit strains. We will gradually further improve the data to achieve regularly updated database of animal strains in the future. Discussions The establishment of this database will facilitate scientific researchers in our country to access and use domestic and foreign resources of laboratory animal strains, and provide links to introduce laboratory animal strain resources from European, American and other developed countries.