慢性肠道炎症促进肝癌的发展
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973课题"细胞微环境在肿瘤发生发展及侵袭转移中的作用及机制"(2011CB504205)。


Chronic colitis induced by irritant dextran sodium sulphate promote hepatoma development in mice
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    摘要:

    目的 探讨慢性肠道炎症状态下菌群结构及构成改变对肝癌进展的影响。方法 22只雄性乙肝病毒转基因小鼠在出生后第2周腹腔注射二乙基亚硝胺诱导小鼠原发性肝癌, 随机分为慢性肠炎组(给予含2%葡聚糖硫酸钠的饮用水)和对照组(给予正常饮用水)。上述小鼠观察至22周龄。在此期间定期进行肝功能测定, 收集粪便进行宏基因组测序。另将4只已发生肝癌的小鼠与7只8周龄肉眼及病理学未见肝癌的小鼠同笼(分2笼)饲养16周。结果 DSS所诱导的慢性肠炎小鼠与正常饮用水小鼠相比肝功能无显著改变;DSS诱导的慢性肠炎组小鼠(n=10)在第22周龄时有9只发生弥漫性肉眼可见的肝癌, 而正常饮用水小鼠(n=12)仅有2只发生肝癌, 肿瘤发生率(P=0.002)和肿瘤数量(P=0.028)有显著差别;与正常饮用水小鼠相比, 慢性肠炎组小鼠肠道菌群中, 普雷沃氏菌属(P=0.022)和厌氧支原体属(P=0.014)显著减少;与已发生肝癌小鼠进行同笼饲养的小鼠(n=7)在24周龄时全部发生肉眼可见的肝癌。结论 慢性肠道炎症状态下, 肠道菌群改变可促进肝癌进展。

    Abstract:

    Objective To investigate the effect of gut bacteria under chronic colitis on the progression of hepatoma in mice. Methods 22 hepatitis B virus (HBV)-transgenic mice (male, 8 weeks) were randomly divided into two groups, one group (n=10) was fed the drinking water containing 2% dextran sodium sulphate(DSS)to induce chronic colitis and the control group(n=12)was fed with normal drinking water. In order to investigate the effect of gut microbes, 7 male HBV-transgenic mice(8 weeks, with no detectable hepatoma under microscopy) were cohoused with 4 mice with hepatoma for 16 weeks. Results No significant liver cell damage was observed in the group of the mice fed with 2% DSS-containing drinking water. By the 22-week old, 9 of the 10 mice(90.0%) fed with 2% DSS-containing drinking water, 2 of the 12 mice(16.7%) fed with normal drinking had hepatoma. Both the hepatoma incidence and the tumor numbers in the group of mice fed with DSS-containing water were significantly higher than that in the controls (P=0.002 and P=0.028respetively). Compared to controls, the bacteria family Prevotella (P=0.022) and Anaeroplasma (P=0.014) reduced significantly in the mice with induced chronic colitis. All the mice (n=7) cohoused with the mice with hepatoma had the liver tumor developed at 24-week-old. Conclusion Alterations of gut bacteria under chronic colitis may promote the development of liver cancer.

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易宏淦,臧梦雅,吴志远,朱正,赵新明,王维虎,曲春枫.慢性肠道炎症促进肝癌的发展[J].中国比较医学杂志,2015,25(5):1~4.

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  • 最后修改日期:2015-03-31
  • 在线发布日期: 2015-05-29
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