Animal experiments in pathogen studies such as avian influenza viruses are increasing in recent years. Different Animal Biosafety Level (ABSL) facilities have been established completely according to the national standards for biosafety. However, issues of personal protection, experiment management, risk recognition and control, animal care with infectious pathogens, etc. should be strengthened. In this review, suggestions and comments are proposed, and hopefully, are useful for related guideline compilations.